Procedures Performed at the Vita In Vitro Centre

Information

IUI - Intrauterine Insemination (Intra Uterine Insemination)

• Timing of ovulation with the help of ultrasound examinations
• Processing of the partner's seminal fluid in laboratory conditions with the aim of extracting and concentrating the highest quality sperm cells
• Placement of the processed sperm cells with a small amount of culture medium directly into the uterus using a very fine catheter

Conception with IUI occurs naturally in the woman's body.

IVF - Classic In Vitro Fertilisation (In Vitro Fertilization)

• Ovarian stimulation with gonadotropic hormones and its monitoring with ultrasound examinations
• Follicular puncture
• Fertilisation of the aspirated eggs with processed sperm cells from the partner in laboratory conditions
• Cultivation of the obtained embryos
• Embryo transfer
• Medicinal support of the luteal phase

ICSI - Intracytoplasmic Sperm Injection (Intra Cytoplasmic Sperm Injection)

• All procedures listed for classic in vitro fertilisation
• A variant of laboratory fertilisation of the egg, in which a single sperm cell is injected into its cytoplasm using a micro-needle

IVM - In Vitro Maturation (In Vitro Maturation)

• A variant of the in vitro procedure in which hormonal stimulation is not applied
• Follicular puncture at an earlier stage of the menstrual cycle and aspiration of immature eggs
• Cultivation of the immature eggs in laboratory conditions for their maturation
• Fertilisation of the matured eggs using standard IVF/ICSI procedures
• Cultivation of the obtained embryos
• Embryo transfer
• Medicinal support of the luteal phase

Microsurgical techniques for sperm retrieval in severe cases of male infertility

Performed by a urologist under appropriate anaesthesia. Depending on the location and method of sperm extraction, the following techniques are used:

• PESA - Percutaneous Epididymal Sperm Aspiration
• MESA - Microsurgical Epididymal Sperm Aspiration
• TESA - Testicular Sperm Aspiration
• TESE - Testicular Sperm Extraction

Cryopreservation of eggs, sperm cells, and embryos

• Freezing of eggs, sperm cells, and embryos
• Storage at -196 degrees
• Thawing and use for IVF, ICSI, or IUI